pMT[Hygro]_GBP-3xFlag-DEST
(Plasmid #157813)

• Purpose: A gateway plasmid for expression of GBP-3xFlag-fusion protein under the regulation of the copper-inducible metallothionein promoter in Drosophila cells; suitable for hygromycin selection
• Depositing Lab: Zoltan Lipinszki
• Publication: Torras-Llort et al Commun Biol. 2020 Aug 19;3(1):454. doi: 10.1038/s42003-020-01182-y.

Backbone

• Vector backbone: pMT[Hygro]_GBP-3xFlag-DEST
• Backbone manufacturer: -
• Vector type: Insect Expression ; For Drosophila cells
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol and Ampicillin, 25 & 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): ccdB Survival
• Growth instructions: After LR reaction transform DH5alpha cells and grow Ampicillin-containing plates at 37C
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: GFP-binding protein
• Alt name: GBP
• Alt name: GFP nanobody
• Species: Lama glama
• Insert Size (bp): 351
• Promoter: metallothionein
• Tag / Fusion Protein:
  • 3xFlag (C terminal on insert)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: a
• 3′ sequencing primer: a

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry