pJDC60
(Plasmid
#157780)
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PurposetdTomato is expressed under the mycobacterial L5 promoter for in vivo and in vitro detection.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 157780 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepJDC89
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nametdTomato
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SpeciesSynthetic
- Promoter L5
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (unknown if destroyed)
- 3′ cloning site KpnI (unknown if destroyed)
- 5′ sequencing primer F: 5’-CAAGCTGAAGGTGACCAAGG-3’
- 3′ sequencing primer 5’-GTGATGAACTTCGAGGACGG-3’ (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byDr. Rodger Y. Tsien
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pJDC60 was a gift from Jeffrey Cirillo (Addgene plasmid # 157780 ; http://n2t.net/addgene:157780 ; RRID:Addgene_157780) -
For your References section:
Fluorescence Imaging of Mycobacterial Infection in Live Mice Using Fluorescent Protein-Expressing Strains. Kong Y, Cirillo JD. Methods Mol Biol. 2018;1790:75-85. doi: 10.1007/978-1-4939-7860-1_6. 10.1007/978-1-4939-7860-1_6 PubMed 29858784
Map uploaded by the depositor.
