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Drosophila Nedd2-like caspase (DRONC)
(Plasmid #157778)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 157778 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET23b
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 3666
  • Total vector size (bp) 5066
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Drosophila Nedd2-like caspase (DRONC)
  • Alt name
    DRONC
  • Species
    D. melanogaster (fly)
  • Insert Size (bp)
    1400
  • Entrez Gene
    Dronc (a.k.a. Dmel_CG8091, CG8090, CG8091, DRONC, Dmel\CG8091, Dronc/Casp9, Nc, Nc\Dronc, anon-EST:GreesD6, anon-EST:GressD6, anon-WO0118547.313, caspase-9, dronc)
  • Promoter T7
  • Tag / Fusion Protein
    • 6x HIS (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (unknown if destroyed)
  • 3′ cloning site XhoI (unknown if destroyed)
  • 5′ sequencing primer T7 Promotor
  • 3′ sequencing primer T7 Terminator
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    The plasmid pET23b encoding Drosophila wt DRONC plus a C-terminal His6 purification tag was a generous gift from Dr Rollie Clem.

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Drosophila Nedd2-like caspase (DRONC) was a gift from Guy Salvesen (Addgene plasmid # 157778 ; http://n2t.net/addgene:157778 ; RRID:Addgene_157778)

  • For your References section:

    Activation mechanism and substrate specificity of the Drosophila initiator caspase DRONC. Snipas SJ, Drag M, Stennicke HR, Salvesen GS. Cell Death Differ. 2008 May;15(5):938-45. doi: 10.1038/cdd.2008.23. Epub 2008 Feb 29. 10.1038/cdd.2008.23 PubMed 18309328
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