pCMV mTurquoise2-shadowY tandem
(Plasmid
#157775)
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Purpose(Empty Backbone) Produces a fusion between mTurquoise2 and shadowY. It can be used as a positive control for FRET (using FLIM)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 157775 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCMV mTurquoise2
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Backbone manufacturerTramier
- Backbone size (bp) 4741
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Vector typeMammalian Expression
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Tags
/ Fusion Proteins
- mTurquoise2 (N terminal on insert)
- shadowY (C terminal on backbone)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNone
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Tags
/ Fusion Proteins
- mTurquoise2 (N terminal on insert)
- shadowY (C terminal on backbone)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCMV mTurquoise2-shadowY tandem was a gift from Marc Tramier (Addgene plasmid # 157775 ; http://n2t.net/addgene:157775 ; RRID:Addgene_157775) -
For your References section:
Aurora kinase A localises to mitochondria to control organelle dynamics and energy production. Bertolin G, Bulteau AL, Alves-Guerra MC, Burel A, Lavault MT, Gavard O, Le Bras S, Gagne JP, Poirier GG, Le Borgne R, Prigent C, Tramier M. eLife. 2018 Aug 2;7. pii: 38111. doi: 10.7554/eLife.38111. 10.7554/eLife.38111 PubMed 30070631