pTarget TMEM97-P2A-mCardinal
(Plasmid #157747)

• Purpose: Express in mammalian cells TMEM97 and mCardianl to follow transfection efficiency using flow cytometry. mCardinal is expressed following a P2A skip peptide so it is not connected to TMEM97.
• Depositing Lab: Andrew Kruse
• Publication: Alon et al Proc Natl Acad Sci U S A. 2017 Jul 3;114(27):7160-7165. doi: 10.1073/pnas.1705154114. Epub 2017 May 30.

Backbone

• Vector backbone: pTarget
• Backbone manufacturer: Promega
• Backbone size w/o insert (bp): 5670
• Vector type: Mammalian Expression
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: TMEM97
• Alt name: mCardinal
• Species: H. sapiens (human)
• Insert Size (bp): 1302
• Entrez Gene: TMEM97 (a.k.a. MAC30)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: unknown

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Industry MTA