Plasmid EUPp_09
(Plasmid #157745)

• Purpose: PgyrA_ada _adh2 _pMB1_ampR (for expressing two enzymes (Ada and Adh2) to allow E. coli to consume ethanol)
• Depositing Lab: Kang Zhou
• Publication: Liang et al Metab Eng. 2020 Nov 25. pii: S1096-7176(20)30180-4. doi: 10.1016/j.ymben.2020.11.010.

Backbone

• Vector backbone: PgyrA_pMB1_ampR
• Backbone size w/o insert (bp): 2502
• Total vector size (bp): 4952
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: acetaldehyde dehydrogenase
• Alt name: ada
• Species: Dickeya zeae
• Insert Size (bp): 1374
• Promoter: PgyrA

Cloning Information for Gene/Insert 1

• Cloning method: Unknown
• 5′ sequencing primer: ggaacactccgtgatcgaacc
• 3′ sequencing primer: Aggcaatacggaacatatcc

Gene/Insert 2

• Gene/Insert name: alcohol dehydrogenase
• Alt name: adh2
• Species: S. cerevisiae (budding yeast)
• Insert Size (bp): 1041
• GenBank ID:
• Entrez Gene: ADH2 (a.k.a. YMR303C, ADR2)
• Promoter: PgyrA

Cloning Information for Gene/Insert 2

• Cloning method: Unknown
• 5′ sequencing primer: Gtctattccagaaactcaaaa
• 3′ sequencing primer: Atttagaagtgtcaacaacg

Resource Information

• Supplemental Documents:
  • PgyrA_ada _adh2 _pMB1_ampR.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry