pcDNA5-FRT-TO-FH-Nsp13_GC3opt
(Plasmid #157715)

• Purpose: mammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Nsp13 under control of a tetracycline-inducible promoter
• Depositing Lab: David Tollervey
• Publication: Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi: 10.12688/wellcomeopenres.16322.1. eCollection 2020.

Backbone

• Vector backbone: pcDNA5-FRT-TO
• Backbone manufacturer: Thermo Fisher
• Vector type: Mammalian Expression
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: FH-Nsp13_GC3opt
• Species: SARS-CoV-2
• Mutation: codon optimized; gene expression was optimized by increasing the GC3 content within codons
• Entrez Gene: ORF1ab (a.k.a. GU280_gp01)
• Tag / Fusion Protein:
  • Flag-His8 (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Unknown (unknown if destroyed)
• 3′ cloning site: Unknown (unknown if destroyed)

Resource Information

• A portion of this plasmid was derived from a plasmid made by: IDT

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Industry MTA