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pcDNA5-FRT-TO-Nsp8-Nsp7-HF_GC3opt
(Plasmid #157702)

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Item Catalog # Description Quantity Price (USD)
Plasmid 157702 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    pcDNA5-FRT-TO
  • Backbone manufacturer
    Thermo Fisher
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Nsp8-Nsp7-HF_GC3opt
  • Species
    SARS-CoV-2
  • Mutation
    codon optimized; gene expression was optimized by increasing the GC3 content within codons
  • Entrez Gene
    ORF1ab (a.k.a. GU280_gp01)
  • Tag / Fusion Protein
    • His8-Flag (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Gene Art

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA5-FRT-TO-Nsp8-Nsp7-HF_GC3opt was a gift from David Tollervey (Addgene plasmid # 157702 ; http://n2t.net/addgene:157702 ; RRID:Addgene_157702)

  • For your References section:

    Integrative vectors for regulated expression of SARS-CoV-2 proteins implicated in RNA metabolism. Bresson S, Robertson N, Sani E, Turowski TW, Shchepachev V, Kompauerova M, Spanos C, Helwak A, Tollervey D. Wellcome Open Res. 2020 Nov 3;5:261. doi: 10.12688/wellcomeopenres.16322.1. eCollection 2020. 10.12688/wellcomeopenres.16322.1 PubMed 33313418
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