pcDNA5-FRT-TO-N-Flag-8His
(Plasmid
#157677)
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PurposeN-terminal tagging vector
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 157677 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * | |
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Backbone
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Vector backbonepcDNA5-FRT-TO
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Backbone manufacturerThermo Fisher
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Vector typeMammalian Expression
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameFlag-8His
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SpeciesSynthetic
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
- 3′ cloning site Unknown (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA5-FRT-TO-N-Flag-8His was a gift from David Tollervey (Addgene plasmid # 157677 ; http://n2t.net/addgene:157677 ; RRID:Addgene_157677) -
For your References section:
Integrative vectors for regulated expression of SARS-CoV-2 proteins implicated in RNA metabolism. Bresson S, Robertson N, Sani E, Turowski TW, Shchepachev V, Kompauerova M, Spanos C, Helwak A, Tollervey D. Wellcome Open Res. 2020 Nov 3;5:261. doi: 10.12688/wellcomeopenres.16322.1. eCollection 2020. 10.12688/wellcomeopenres.16322.1 PubMed 33313418
