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Addgene

pQE80L-His-SpyTag-XMod-Doc(WT)
(Plasmid #157675)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 157675 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pQE80l
  • Backbone size w/o insert (bp) 4500
  • Total vector size (bp) 5513
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    XMod-Doc Wild type with Histag and SpyTag
  • Insert Size (bp)
    993
  • Promoter T5
  • Tag / Fusion Protein
    • Histag and SpyTag

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer CGAAGCTCGACGGCCACAATACCGTTACCAGCGCAGTT
  • 3′ sequencing primer TGTTAGCAGCCGGATCCTTATTCTTCTTCCGCATCACCTG
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pQE80L-His-SpyTag-XMod-Doc(WT) was a gift from Michael Nash (Addgene plasmid # 157675 ; http://n2t.net/addgene:157675 ; RRID:Addgene_157675)

  • For your References section:

    Influence of Fluorination on Single-Molecule Unfolding and Rupture Pathways of a Mechanostable Protein Adhesion Complex. Yang B, Liu H, Liu Z, Doenen R, Nash MA. Nano Lett. 2020 Nov 16. doi: 10.1021/acs.nanolett.0c04178. 10.1021/acs.nanolett.0c04178 PubMed 33191756
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