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pET28a-Coh-ddFLN4-ELP(MV7E2)3-His-ybbR
(Plasmid #157672)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 157672 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET28a
  • Backbone size w/o insert (bp) 5200
  • Total vector size (bp) 6740
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Cohesin (R.f) with ddFLN4, ELP, Histag and ybbR tag
  • Insert Size (bp)
    1518
  • Promoter T7
  • Tag / Fusion Protein
    • ddFLN4, ELP, Histag and ybbR tag

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer GAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGGCGCTCACAGACAGAGGAA
  • 3′ sequencing primer CCGGGTCAGCAGAACCGGATCCAGAACCGGAGCCTGGCTCACCAGCCT
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET28a-Coh-ddFLN4-ELP(MV7E2)3-His-ybbR was a gift from Michael Nash (Addgene plasmid # 157672 ; http://n2t.net/addgene:157672 ; RRID:Addgene_157672)

  • For your References section:

    Influence of Fluorination on Single-Molecule Unfolding and Rupture Pathways of a Mechanostable Protein Adhesion Complex. Yang B, Liu H, Liu Z, Doenen R, Nash MA. Nano Lett. 2020 Nov 16. doi: 10.1021/acs.nanolett.0c04178. 10.1021/acs.nanolett.0c04178 PubMed 33191756
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