shFGF2#2
(Plasmid
#157668)
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PurposeTet inducible knockdown of FGF2
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 157668 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneTet-pLKO-puro
- Backbone size w/o insert (bp) 8800
- Total vector size (bp) 8860
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Vector typeMammalian Expression, Lentiviral, RNAi
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameFibroblast growth factor 2
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Alt nameshFGF2#2
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SpeciesH. sapiens (human)
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Insert Size (bp)60
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Entrez GeneFGF2 (a.k.a. BFGF, FGF-2, FGFB, HBGF-2)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site AgeI (unknown if destroyed)
- 3′ cloning site EcoRI (unknown if destroyed)
- 5′ sequencing primer -
- 3′ sequencing primer - (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
shFGF2#2 was a gift from Byung Il Lee (Addgene plasmid # 157668 ; http://n2t.net/addgene:157668 ; RRID:Addgene_157668) -
For your References section:
Regulation of mRNA export through API5 and nuclear FGF2 interaction. Bong SM, Bae SH, Song B, Gwak H, Yang SW, Kim S, Nam S, Rajalingam K, Oh SJ, Kim TW, Park S, Jang H, Lee BI. Nucleic Acids Res. 2020 Jun 19;48(11):6340-6352. doi: 10.1093/nar/gkaa335. 10.1093/nar/gkaa335 PubMed 32383752
Map uploaded by the depositor.
