API5-CRISPR
(Plasmid #157664)

• Purpose: Expresses Cas9 and sgRNA targeting API5
• Depositing Lab: Byung Il Lee
• Publication: Bong et al Nucleic Acids Res. 2020 Jun 19;48(11):6340-6352. doi: 10.1093/nar/gkaa335.

Backbone

• Vector backbone: lentiCRISPR v2
• Backbone size w/o insert (bp): 13000
• Total vector size (bp): 13025
• Vector type: Mammalian Expression, Lentiviral, CRISPR
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Apoptosis inhibitor 5
• Alt name: API5
• Species: H. sapiens (human)
• Insert Size (bp): 25
• GenBank ID: NM_001142930 NM_001142930
• Entrez Gene: API5 (a.k.a. AAC-11, AAC11)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BsmBI (unknown if destroyed)
• 3′ cloning site: BsmBI (unknown if destroyed)
• 5′ sequencing primer: -
• 3′ sequencing primer: -

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry