pGEX-UAP56
(Plasmid #157661)

• Purpose: Expresses UAP56 in E.coli cell
• Depositing Lab: Byung Il Lee
• Publication: Bong et al Nucleic Acids Res. 2020 Jun 19;48(11):6340-6352. doi: 10.1093/nar/gkaa335.

Backbone

• Vector backbone: PGEX-4T-3
• Backbone size w/o insert (bp): 4900
• Total vector size (bp): 6058
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Spliceosome RNA helicase DDX39B
• Alt name: UAP56
• Species: H. sapiens (human)
• Insert Size (bp): 1158
• Mutation: deleted amino acids 1-43
• GenBank ID: NM_004640
• Entrez Gene: DDX39B (a.k.a. BAT1, D6S81E, UAP56)
• Tag / Fusion Protein:
  • GST tag (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (unknown if destroyed)
• 3′ cloning site: XhoI (unknown if destroyed)
• 5′ sequencing primer: -
• 3′ sequencing primer: -

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry