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Addgene

pGEX-API5
(Plasmid #157660)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 157660 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGEX-4T-3
  • Backbone size w/o insert (bp) 4900
  • Total vector size (bp) 6412
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Apoptosis inhibitor 5
  • Alt name
    API5
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1512
  • GenBank ID
    NM_001142930
  • Entrez Gene
    API5 (a.k.a. AAC-11, AAC11)
  • Tag / Fusion Protein
    • GST tag (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (unknown if destroyed)
  • 3′ cloning site NotI (unknown if destroyed)
  • 5′ sequencing primer -
  • 3′ sequencing primer -
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEX-API5 was a gift from Byung Il Lee (Addgene plasmid # 157660 ; http://n2t.net/addgene:157660 ; RRID:Addgene_157660)

  • For your References section:

    Regulation of mRNA export through API5 and nuclear FGF2 interaction. Bong SM, Bae SH, Song B, Gwak H, Yang SW, Kim S, Nam S, Rajalingam K, Oh SJ, Kim TW, Park S, Jang H, Lee BI. Nucleic Acids Res. 2020 Jun 19;48(11):6340-6352. doi: 10.1093/nar/gkaa335. 10.1093/nar/gkaa335 PubMed 32383752
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