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Addgene

GGDestX2-Amp
(Plasmid #157654)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 157654 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pX330
  • Backbone manufacturer
    Feng Zhang lab
  • Backbone size (bp) 4267
  • Modifications to backbone
    pX330 was linearized with BbsI. A dsOligo insert (BsaI, BsaI) with complementary 4 bp overhangs was ligated into the pX330 backbone. This construct was linearized with AgeI and SacI to remove the Cas open reading frame and replace it with a BbsI, BbsI dsOligo insert.
  • Vector type
    Mammalian Expression, Synthetic Biology
  • Promoter U6, CBh

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The annotated sequence is also available at the Haynes lab Benchling site: https://benchling.com/s/seq-a5tlIDUJ212rLoJgH6yR

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GGDestX2-Amp was a gift from Karmella Haynes (Addgene plasmid # 157654 ; http://n2t.net/addgene:157654 ; RRID:Addgene_157654)

  • For your References section:

    Rapid Single-Pot Assembly of Modular Chromatin Proteins for Epigenetic Engineering. Priode JH, Haynes KA. Protocols.io, Jan 19, 2021 10.17504/protocols.io.brgcm3sw
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