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Addgene

GGDestX1-Amp
(Plasmid #157652)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 157652 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pTXTL-P70a(2)-deGFP
  • Backbone manufacturer
    Arbor Biosciences
  • Backbone size (bp) 2536
  • Modifications to backbone
    The deGFP open reading frame between the start and stop codons was replaced by a BbsI-deGFP-BbsI drop-in site. Four BbsI sites in the backbone were eliminated by T > A mutations.
  • Vector type
    Bacterial Expression ; Cell-free Expression (E. coli sigma-70)
  • Promoter OR2-OR1-Pr

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The annotated sequence is also available at the Haynes lab Benchling site: https://benchling.com/s/seq-4Syx826a0gonKiH8Evvg.
Please visit https://doi.org/10.17504/protocols.io.brgcm3sw for the protocol.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GGDestX1-Amp was a gift from Karmella Haynes (Addgene plasmid # 157652 ; http://n2t.net/addgene:157652 ; RRID:Addgene_157652)

  • For your References section:

    Rapid Single-Pot Assembly of Modular Chromatin Proteins for Epigenetic Engineering. Haynes KA, Priode JH. Methods Mol Biol. 2023;2599:191-214. doi: 10.1007/978-1-0716-2847-8_14. 10.1007/978-1-0716-2847-8_14 PubMed 36427151
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