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Addgene

GGDest2-Amp
(Plasmid #157650)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 157650 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    V0120
  • Backbone size (bp) 3280
  • Modifications to backbone
    V0120 was linearized with EcoRI and PstI. A dsOligo insert (BamHI, BbsI, BbsI, HindIII) with EcoRI and PstI overhangs was ligated into the V0120 backbone. V0120 (aka V0100) was derived from pUC19 (C. Ajo-Franklin, Pam Silver lab, Harvard).
  • Vector type
    Synthetic Biology
  • Promoter none

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ sequencing primer M13 forward (5'-GTAAAACGACGGCCAG)
  • 3′ sequencing primer M13 reverse (5'-CAGGAAACAGCTATGAC)
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The annotated sequence is also available at the Haynes lab Benchling site: https://benchling.com/s/seq-IBDVQps5oOZ1KXXrxxIx.
Please visit https://doi.org/10.17504/protocols.io.brgcm3sw for the protocol.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GGDest2-Amp was a gift from Karmella Haynes (Addgene plasmid # 157650 ; http://n2t.net/addgene:157650 ; RRID:Addgene_157650)

  • For your References section:

    Rapid Single-Pot Assembly of Modular Chromatin Proteins for Epigenetic Engineering. Haynes KA, Priode JH. Methods Mol Biol. 2023;2599:191-214. doi: 10.1007/978-1-0716-2847-8_14. 10.1007/978-1-0716-2847-8_14 PubMed 36427151
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