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Addgene

pDH010
(Plasmid #157645)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 157645 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET28b
  • Total vector size (bp) 7833
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert 1

  • Gene/Insert name
    sMHT(1-113)-GRX2_C37A

Gene/Insert 2

  • Gene/Insert name
    GRX2_C37A-sMHT(114-230)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note that the Addgene verified sequence differs from the depositor reference sequence. There is no HA-tag on fused to GRX2_C37A-sMHT(114-230). These differences otherwise do not impact overall plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDH010 was a gift from Jonathan Silberg (Addgene plasmid # 157645 ; http://n2t.net/addgene:157645 ; RRID:Addgene_157645)

  • For your References section:

    A Split Methyl Halide Transferase AND Gate That Reports by Synthesizing an Indicator Gas. Fulk EM, Huh D, Atkinson JT, Lie M, Masiello CA, Silberg JJ. ACS Synth Biol. 2020 Nov 20;9(11):3104-3113. doi: 10.1021/acssynbio.0c00355. Epub 2020 Oct 26. 10.1021/acssynbio.0c00355 PubMed 33104325
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