pEMF034
(Plasmid
#157631)
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PurposesMHT-113
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 157631 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneColE1
- Total vector size (bp) 5012
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert 1
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Gene/Insert namesMHT(1-113)-SYNZIP17
Gene/Insert 2
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Gene/Insert nameSYNZIP18-sMHT(114-230)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEMF034 was a gift from Jonathan Silberg (Addgene plasmid # 157631 ; http://n2t.net/addgene:157631 ; RRID:Addgene_157631) -
For your References section:
A Split Methyl Halide Transferase AND Gate That Reports by Synthesizing an Indicator Gas. Fulk EM, Huh D, Atkinson JT, Lie M, Masiello CA, Silberg JJ. ACS Synth Biol. 2020 Nov 20;9(11):3104-3113. doi: 10.1021/acssynbio.0c00355. Epub 2020 Oct 26. 10.1021/acssynbio.0c00355 PubMed 33104325