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pCMV-FLAG TIF1gamma M (456-887)
(Plasmid #15735)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 15735 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCMV5 modified to contain a FLAG epitope
  • Backbone size w/o insert (bp) 4600
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TIF1 gamma M
  • Alt name
    Transcriptional Intermediary Factor 1 gamma
  • Alt name
    TIF1g
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1293
  • Mutation
    Middle region (amino acids 456-887).
  • Entrez Gene
    TRIM33 (a.k.a. ECTO, PTC7, RFG7, TF1G, TIF1G, TIF1GAMMA, TIFGAMMA)
  • Tag / Fusion Protein
    • Flag (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII/BamHI (destroyed during cloning)
  • 3′ cloning site XhoI/SalI (destroyed during cloning)
  • 5′ sequencing primer CMV-F
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The indicated coding region was amplified from cDNA templates by polymerase chain reaction (PCR), then subsequently digested with BamHI/XhoI and inserted into the BglII/SalI sites of a modified pCMV5 plasmid containing one copy of Flag epitope.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV-FLAG TIF1gamma M (456-887) was a gift from Joan Massague (Addgene plasmid # 15735 ; http://n2t.net/addgene:15735 ; RRID:Addgene_15735)

  • For your References section:

    Hematopoiesis controlled by distinct TIF1gamma and Smad4 branches of the TGFbeta pathway. He W, Dorn DC, Erdjument-Bromage H, Tempst P, Moore MA, Massague J. Cell. 2006 Jun 2. 125(5):929-41. 10.1016/j.cell.2006.03.045 PubMed 16751102
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