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Addgene

pGEX Smad2 (L+MH2)
(Plasmid #15730)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 15730 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGEX-4T1
  • Backbone manufacturer
    Amersham
  • Backbone size w/o insert (bp) 4969
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Smad2 (L+MH2)
  • Alt name
    Smad2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    840
  • Mutation
    amino acids 187-467 (linker and MH2 regions).
  • Entrez Gene
    SMAD2 (a.k.a. CHTD8, JV18, JV18-1, LDS6, MADH2, MADR2, hMAD-2, hSMAD2)
  • Tag / Fusion Protein
    • GST (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer pGEX 5'
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEX Smad2 (L+MH2) was a gift from Joan Massague (Addgene plasmid # 15730 ; http://n2t.net/addgene:15730 ; RRID:Addgene_15730)

  • For your References section:

    Hematopoiesis controlled by distinct TIF1gamma and Smad4 branches of the TGFbeta pathway. He W, Dorn DC, Erdjument-Bromage H, Tempst P, Moore MA, Massague J. Cell. 2006 Jun 2. 125(5):929-41. 10.1016/j.cell.2006.03.045 PubMed 16751102
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