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Addgene

pBABE-puro LIP
(Plasmid #15713)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 15713 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBABE puro
  • Backbone manufacturer
    Available at Addgene (#1764)
  • Backbone size w/o insert (bp) 5169
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    LIP
  • Alt name
    C/EBP beta isoform
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    500
  • Entrez Gene
    CEBPB (a.k.a. C/EBP-beta, IL6DBP, NF-IL6, TCF5)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer pBABE 5'
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Human LIP was cloned by PCR from cDNA template and then digested with EcoR1/SalI and inserted in pBabe-Puro.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBABE-puro LIP was a gift from Joan Massague (Addgene plasmid # 15713 ; http://n2t.net/addgene:15713 ; RRID:Addgene_15713)

  • For your References section:

    C/EBPbeta at the core of the TGFbeta cytostatic response and its evasion in metastatic breast cancer cells. Gomis RR, Alarcon C, Nadal C, Van Poznak C, Massague J. Cancer Cell. 2006 Sep . 10(3):203-14. 10.1016/j.ccr.2006.07.019 PubMed 16959612
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