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pGL2 p15 (-756/+68) SBR2 Mut 3' box
(Plasmid #15711)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 15711 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGL2-E1bTATA
  • Backbone size w/o insert (bp) 6000
  • Vector type
    Mammalian Expression, Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    p15 promoter
  • Alt name
    p15INK4b promoter
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    804
  • Mutation
    Mut 3' box in SBR2. The 3' box contains the C/EBP beta binding element.
  • Entrez Gene
    CDKN2B (a.k.a. CDK4I, INK4B, MTS2, P15, TP15, p15INK4b)

Cloning Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

SBR2 is one of the Smad Binding Regions on the promoter

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGL2 p15 (-756/+68) SBR2 Mut 3' box was a gift from Joan Massague (Addgene plasmid # 15711 ; http://n2t.net/addgene:15711 ; RRID:Addgene_15711)

  • For your References section:

    C/EBPbeta at the core of the TGFbeta cytostatic response and its evasion in metastatic breast cancer cells. Gomis RR, Alarcon C, Nadal C, Van Poznak C, Massague J. Cancer Cell. 2006 Sep . 10(3):203-14. 10.1016/j.ccr.2006.07.019 PubMed 16959612
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