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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 15697 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepFlag-CMV2
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Backbone manufacturerSigma
- Backbone size w/o insert (bp) 4679
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameACAP1
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Alt namecentaurin, beta 1
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SpeciesH. sapiens (human)
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Entrez GeneACAP1 (a.k.a. CENTB1)
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Tag
/ Fusion Protein
- Flag (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NotI (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer CMV-F (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byACAP1/KIAA0050 cDNA was obtained from Dr. Takahiro Nagese at the Kazusa DNA Research Institute (Chiba, Japan)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pFLAG-CMV2 ACAP1 was a gift from Paul Randazzo (Addgene plasmid # 15697 ; http://n2t.net/addgene:15697 ; RRID:Addgene_15697) -
For your References section:
ACAPs are arf6 GTPase-activating proteins that function in the cell periphery. Jackson TR, Brown FD, Nie Z, Miura K, Foroni L, Sun J, Hsu VW, Donaldson JG, Randazzo PA. J Cell Biol. 2000 Oct 30. 151(3):627-38. 10.1083/jcb.151.3.627 PubMed 11062263