pPyCAG-Myc-ERas-IP
(Plasmid #15690)

• Depositing Lab: Shinya Yamanaka
• Publication: Takahashi et al Nature. 2003 May 29. 423(6939):541-5.

Backbone

• Vector backbone: pPyCAG-IP
• Backbone size w/o insert (bp): 6800
• Vector type: Mammalian Expression
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: ERas
• Species: M. musculus (mouse)
• Insert Size (bp): 700
• Entrez Gene: Eras (a.k.a. RP23-198C2.4, HRAS2, HRasp, Ha-Ras2, ecat5)
• Tag / Fusion Protein:
  • Myc (6x) (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: attB1 (unknown if destroyed)
• 3′ cloning site: attB2 (unknown if destroyed)
• 5′ sequencing primer: CAG-1

Resource Information

• A portion of this plasmid was derived from a plasmid made by: CAG promoter was from Dr. Jun-ichi Miyazaki of Osaka University Graduate School of Medicine. In publication using this plasmid, please cite: Efficient selection for high-expression transfectants with a novel eukaryotic vector. Gene 108:193-200, 1991. Niwa, H., Yamamura, K. & Miyazaki, J.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry