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Addgene

pCAG-ERas-IH
(Plasmid #15688)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 15688 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCAG-IH
  • Backbone size w/o insert (bp) 6400
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ERas
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    700
  • Entrez Gene
    Eras (a.k.a. RP23-198C2.4, HRAS2, HRasp, Ha-Ras2, ecat5)

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ cloning site attB1 (unknown if destroyed)
  • 3′ cloning site Gateway cloning (unknown if destroyed)
  • 5′ sequencing primer attB2
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    CAG promoter was from Dr. Jun-ichi Miyazaki of Osaka University Graduate School of Medicine. In publication using this plasmid, please cite: Efficient selection for high-expression transfectants with a novel eukaryotic vector. Gene 108:193-200, 1991. Niwa, H., Yamamura, K. & Miyazaki, J.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCAG-ERas-IH was a gift from Shinya Yamanaka (Addgene plasmid # 15688 ; http://n2t.net/addgene:15688 ; RRID:Addgene_15688)

  • For your References section:

    Role of ERas in promoting tumour-like properties in mouse embryonic stem cells. Takahashi K, Mitsui K, Yamanaka S. Nature. 2003 May 29. 423(6939):541-5. 10.1038/nature01646 PubMed 12774123
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