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Addgene

pET14b PHAS-I
(Plasmid #15679)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 15679 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET14b
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 4671
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PHAS-I
  • Alt name
    4EBP1
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    800
  • Mutation
    Nucleotides 1-354 of rat PHAS-I plus 450bp of 3'UTR
  • GenBank ID
    NM_053857
  • Entrez Gene
    Eif4ebp1 (a.k.a. PHAS-I)
  • Tag / Fusion Protein
    • 6xHis (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Full-length PHASI with a His tag. See author's map for detailed information.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET14b PHAS-I was a gift from John Lawrence (Addgene plasmid # 15679 ; http://n2t.net/addgene:15679 ; RRID:Addgene_15679)

  • For your References section:

    Two motifs in the translational repressor PHAS-I required for efficient phosphorylation by mammalian target of rapamycin and for recognition by raptor. Choi KM, McMahon LP, Lawrence JC. J Biol Chem. 2003 May 30. 278(22):19667-73. 10.1074/jbc.M301142200 PubMed 12665511
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