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Addgene

pcDNA3 AU1 mTOR S2035W
(Plasmid #15678)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 15678 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5400
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mTOR
  • Alt name
    FRAP1
  • Alt name
    FRAP2
  • Alt name
    RAFT1
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    8554
  • Mutation
    S2035W
  • GenBank ID
    NM_019906
  • Entrez Gene
    Mtor (a.k.a. Frap1, RAFT1)
  • Tag / Fusion Protein
    • AU1 (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Robert T. Abraham (Burnham Institute of Medical Research)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

See author's map for detailed information.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3 AU1 mTOR S2035W was a gift from John Lawrence (Addgene plasmid # 15678 ; http://n2t.net/addgene:15678 ; RRID:Addgene_15678)

  • For your References section:

    The rapamycin-binding domain governs substrate selectivity by the mammalian target of rapamycin. McMahon LP, Choi KM, Lin TA, Abraham RT, Lawrence JC. Mol Cell Biol. 2002 Nov . 22(21):7428-38. 10.1128/MCB.22.21.7428-7438.2002 PubMed 12370290
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