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Addgene

HA PRAS40 P185A
(Plasmid #15675)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 15675 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5400
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PRAS40 P185A
  • Alt name
    PRAS40
  • Alt name
    Akt1S
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    771
  • Mutation
    P185A
  • GenBank ID
    NM_032375
  • Entrez Gene
    AKT1S1 (a.k.a. Lobe, PRAS40)
  • Tag / Fusion Protein
    • HA (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Richard A. Roth (Stanford University)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    HA PRAS40 P185A was a gift from John Lawrence (Addgene plasmid # 15675 ; http://n2t.net/addgene:15675 ; RRID:Addgene_15675)

  • For your References section:

    PRAS40 regulates mTORC1 kinase activity by functioning as a direct inhibitor of substrate binding. Wang L, Harris TE, Roth RA, Lawrence JC. J Biol Chem. 2007 Jul 6. 282(27):20036-44. 10.1074/jbc.M702376200 PubMed 17510057
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