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Addgene

PRAS40 (mouse) shRNA
(Plasmid #15672)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 15672 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLKO.1
  • Backbone manufacturer
    Available at Addgene (#8453)
  • Backbone size w/o insert (bp) 7032
  • Vector type
    Mammalian Expression, Lentiviral, RNAi
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PRAS40 shRNA
  • Alt name
    Akt1S shRNA
  • Alt name
    PRAS40
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    59
  • GenBank ID
    NM_026270
  • Entrez Gene
    Akt1s1 (a.k.a. 1110012J22Rik, AI227026, Lobe, Lobel, PRAS40)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AgeI (not destroyed)
  • 3′ cloning site EcoRI (destroyed during cloning)
  • 5′ sequencing primer pLKO.1 5'
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Oligonucleotides encoding the short-hairpin RNA expression cassette targeting mouse PRAS40 from base 753-773 (GI:124376717) were annealed and subcloned into pLKO.1. See author's map for shRNA sequence information.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PRAS40 (mouse) shRNA was a gift from John Lawrence (Addgene plasmid # 15672 ; http://n2t.net/addgene:15672 ; RRID:Addgene_15672)

  • For your References section:

    PRAS40 regulates mTORC1 kinase activity by functioning as a direct inhibitor of substrate binding. Wang L, Harris TE, Roth RA, Lawrence JC. J Biol Chem. 2007 Jul 6. 282(27):20036-44. 10.1074/jbc.M702376200 PubMed 17510057
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