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Purpose(Empty Backbone)
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 15670 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * | |
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Backbone
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Vector backbonepCM433
- Backbone size (bp) 8081
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Vector typebacterial allelic exchange vector
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol and Ampicillin, 25 & 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNone
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer N/A (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byThis vector was generated by our lab, using a backbone (pCM184) previously developed by the PI (BioTechniques, 33:1062-1067).
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Ampicillin, Chloramphenicol, Tetracycline
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCM433 was a gift from Christopher Marx (Addgene plasmid # 15670 ; http://n2t.net/addgene:15670 ; RRID:Addgene_15670) -
For your References section:
Development of a broad-host-range sacB-based vector for unmarked allelic exchange. Marx CJ. BMC Res Notes. 2008 Feb 26;1:1. doi: 10.1186/1756-0500-1-1. 10.1186/1756-0500-1-1 PubMed 18710539
