DV-GFP-Rab38-WT-pDEST53
(Plasmid
#15669)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 15669 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA-DEST53 Gateway
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 6400
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameRab38
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SpeciesM. musculus (mouse)
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Insert Size (bp)700
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Mutationwildtype Rab38 sequence
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GenBank IDNT_039433
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Entrez GeneRab38 (a.k.a. 2310011F14Rik, cht)
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Tag
/ Fusion Protein
- GFP (N terminal on insert)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer T7 (Common Sequencing Primers)
Resource Information
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Addgene Notes
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
DV-GFP-Rab38-WT-pDEST53 was a gift from William Pavan (Addgene plasmid # 15669 ; http://n2t.net/addgene:15669 ; RRID:Addgene_15669) -
For your References section:
Mutation of melanosome protein RAB38 in chocolate mice. Loftus SK, Larson DM, Baxter LL, Antonellis A, Chen Y, Wu X, Jiang Y, Bittner M, Hammer JA, Pavan WJ. Proc Natl Acad Sci U S A. 2002 Apr 2. 99(7):4471-6. 10.1073/pnas.072087599 PubMed 11917121