-
Depositing Lab
-
Publication
-
Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 15667 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
-
Vector backbonepcDNA3.1 (+)
- Backbone size w/o insert (bp) 5428
-
Vector typeMammalian Expression
-
Selectable markersNeomycin (select with G418)
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert namePACT
-
Alt namePRKRA
-
SpeciesH. sapiens (human)
-
Insert Size (bp)942
-
Entrez GenePRKRA (a.k.a. DYT16, HSD14, PACT, RAX)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer BGH rev (Common Sequencing Primers)
Resource Information
-
Articles Citing this Plasmid
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pcDNA-PACT was a gift from Dong-Yan Jin (Addgene plasmid # 15667 ; http://n2t.net/addgene:15667 ; RRID:Addgene_15667) -
For your References section:
Human TRBP and PACT directly interact with each other and associate with dicer to facilitate the production of small interfering RNA. Kok KH, Ng MH, Ching YP, Jin DY. J Biol Chem. 2007 Jun 15. 282(24):17649-57. 10.1074/jbc.M611768200 PubMed 17452327