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Addgene

pRetrosuper Fbw7 shRNA-1
(Plasmid #15660)

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Item Catalog # Description Quantity Price (USD)
Plasmid 15660 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    pRetrosuper
  • Backbone size w/o insert (bp) 6400
  • Vector type
    Mammalian Expression, Retroviral, RNAi
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Fbw7 shRNA
  • Alt name
    Fbw7
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    64
  • Entrez Gene
    FBXW7 (a.k.a. AGO, CDC4, DEDHIL, FBW6, FBW7, FBX30, FBXO30, FBXW6, SEL-10, SEL10, hAgo, hCdc4)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII? (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer H1
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

hairpin targets the sequence: ACAGGACAGTGTTTACAAA

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRetrosuper Fbw7 shRNA-1 was a gift from Martin Eilers (Addgene plasmid # 15660 ; http://n2t.net/addgene:15660 ; RRID:Addgene_15660)

  • For your References section:

    The ubiquitin-specific protease USP28 is required for MYC stability. Popov N, Wanzel M, Madiredjo M, Zhang D, Beijersbergen R, Bernards R, Moll R, Elledge SJ, Eilers M. Nat Cell Biol. 2007 Jul . 9(7):765-74. 10.1038/ncb1601 PubMed 17558397
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