Skip to main content
Addgene

pETduet-1:MCS1(HemeOxygenase)::MCS2(ReBphP-PCM)
(Plasmid #156463)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 156463 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pET-Duet1
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5420
  • Total vector size (bp) 7620
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    Heme Oxygenase
  • Alt name
    HO
  • Alt name
    biliverdin-producing
  • Species
    Nostocaceae
  • Insert Size (bp)
    723
  • GenBank ID
    BAB73596.1
  • Promoter T7

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site Nco1 (not destroyed)
  • 3′ cloning site PstI (not destroyed)
  • 5′ sequencing primer aaaccatggatggcgatgagc
  • 3′ sequencing primer aaactgcagttactcagcagtggc
    • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    ReBphP-PCM
  • Alt name
    ReBphP
  • Species
    Rhizobium etli
  • Insert Size (bp)
    1572
  • Promoter T7
  • Tag / Fusion Protein
    • His Tag (C terminal on backbone)

Cloning Information for Gene/Insert 2

  • Cloning method Restriction Enzyme
  • 5′ cloning site Nde1 (not destroyed)
  • 3′ cloning site Xho1 (not destroyed)
  • 5′ sequencing primer aaacatatgagcggcaccagag
  • 3′ sequencing primer aaactgatcgccgagctgaacCATCATCACCATCACCACTAA
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

ReBphP was synthesized as gene strings (GeneArt, Life Technologies, Regensburg, Germany) and then cloned in MCS2 of pETDuet1

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pETduet-1:MCS1(HemeOxygenase)::MCS2(ReBphP-PCM) was a gift from Andre Stiel (Addgene plasmid # 156463 ; http://n2t.net/addgene:156463 ; RRID:Addgene_156463)

  • For your References section:

    Multiplexed whole-animal imaging with reversibly switchable optoacoustic proteins. Mishra K, Stankevych M, Fuenzalida-Werner JP, Grassmann S, Gujrati V, Huang Y, Klemm U, Buchholz VR, Ntziachristos V, Stiel AC. Sci Adv. 2020 Jun 12;6(24):eaaz6293. doi: 10.1126/sciadv.aaz6293. eCollection 2020 Jun. 10.1126/sciadv.aaz6293 PubMed 32582850
Related items:
Commonly requested with: