drApo1–T7
(Bacterial strain #156461)

• Purpose: An E. coli strain that expresses an inactive variant of MutaT7. Used as a control strain for mutagenesis experiments with MutaT7.
• Depositing Lab: Matthew D. Shoulders
• Publication: Moore et al J Am Chem Soc. 2018 Sep 19;140(37):11560-11564. doi: 10.1021/jacs.8b04001. Epub 2018 Jul 18.

Backbone

• Vector backbone: None

Growth in Bacteria

• Bacterial Resistance(s): Streptomycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): MutaT7
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: rApoI
• Alt name: rat APOBEC1
• Species: R. norvegicus (rat)
• Mutation: Glutamate 63 is mutated to Glutamine
• Entrez Gene: Apobec1 (a.k.a. REPR, apobec-1)
• Promoter: PA1lacO-Tenth
• Tag / Fusion Protein:
  • T7 RNA polymerase (C terminal on insert)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: gcagaacgcccggcac

Resource Information

• A portion of this plasmid was derived from a plasmid made by: pET28b-BE1 (from David Liu addgene #73018) and pTara (from Kathleen Matthews addgene #31491)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Esherichia coli strain that expresses a deactivated variant of MutaT7, a fusion of rat APOBEC1 and the T7 RNA polymerase. Genotype: DH10B Δung ΔmotAB ΔcsgABCDEFG [PlacI<>Ptac] Δ(araA-leu)7697::[PA1lacO-Tenth drApo1-T7]