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Addgene

pX330-EN1680_Sororin-Cterm
(Plasmid #156451)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 156451 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pX330-EN1680_Sororin-Cterm
  • Vector type
    Mammalian Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Cas9, SORORIN
  • Alt name
    CDC5A
  • Species
    M. musculus (mouse)
  • Mutation
    none
  • Entrez Gene
    Cdca5 (a.k.a. 2610036L13Rik, AL024086, AW536684, C85404)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note: Plasmid contains an A to C single nucleotide mutation in the U6 promoter just upstream of the Sororin gRNA sequence, which did not affect sgRNA activity measurably

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pX330-EN1680_Sororin-Cterm was a gift from Elphege Nora (Addgene plasmid # 156451 ; http://n2t.net/addgene:156451 ; RRID:Addgene_156451)

  • For your References section:

    Molecular basis of CTCF binding polarity in genome folding. Nora EP, Caccianini L, Fudenberg G, So K, Kameswaran V, Nagle A, Uebersohn A, Hajj B, Saux AL, Coulon A, Mirny LA, Pollard KS, Dahan M, Bruneau BG. Nat Commun. 2020 Nov 5;11(1):5612. doi: 10.1038/s41467-020-19283-x. 10.1038/s41467-020-19283-x PubMed 33154377
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