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pKS035 - pCAGGS-3XFLAG-Rad21-mKate2-bpA
(Plasmid #156445)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 156445 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pKS035-pCAGGS-3XFLAG-Rad21-mKate2-bpAk
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mouse RAD21
  • Alt name
    SCC1
  • Species
    M. musculus (mouse)
  • Mutation
    none
  • Entrez Gene
    Rad21 (a.k.a. SCC, SCC1, mHR21, mKIAA0078)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note: The cDNA used of 129/Ola origin contains a natural F374S polymorphism compared to C57BL6.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pKS035 - pCAGGS-3XFLAG-Rad21-mKate2-bpA was a gift from Elphege Nora (Addgene plasmid # 156445 ; http://n2t.net/addgene:156445 ; RRID:Addgene_156445)

  • For your References section:

    Molecular basis of CTCF binding polarity in genome folding. Nora EP, Caccianini L, Fudenberg G, So K, Kameswaran V, Nagle A, Uebersohn A, Hajj B, Saux AL, Coulon A, Mirny LA, Pollard KS, Dahan M, Bruneau BG. Nat Commun. 2020 Nov 5;11(1):5612. doi: 10.1038/s41467-020-19283-x. 10.1038/s41467-020-19283-x PubMed 33154377
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