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pEN313 - Rad21-Halo-Frt-PGK-EM7-NeoR-bpA-Frt targeting
(Plasmid #156431)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 156431 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEN313 - Rad21-Halo-Frt-PGK-EM7-NeoR-bpA-Frt targeting
  • Vector type
    Mammalian Expression, Mouse Targeting

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    RAD21, Halotag
  • Alt name
    SCC1
  • Species
    M. musculus (mouse)
  • Mutation
    none
  • Entrez Gene
    Rad21 (a.k.a. SCC, SCC1, mHR21, mKIAA0078)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEN313 - Rad21-Halo-Frt-PGK-EM7-NeoR-bpA-Frt targeting was a gift from Elphege Nora (Addgene plasmid # 156431 ; http://n2t.net/addgene:156431 ; RRID:Addgene_156431)

  • For your References section:

    Molecular basis of CTCF binding polarity in genome folding. Nora EP, Caccianini L, Fudenberg G, So K, Kameswaran V, Nagle A, Uebersohn A, Hajj B, Saux AL, Coulon A, Mirny LA, Pollard KS, Dahan M, Bruneau BG. Nat Commun. 2020 Nov 5;11(1):5612. doi: 10.1038/s41467-020-19283-x. 10.1038/s41467-020-19283-x PubMed 33154377
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