pSBL_DK202
(Plasmid #156426)

• Purpose: Mammalian expression of 3a delta N(aa 42-275)-EGFP with CMV promoter, can be used for transfection or used to generate bacmids for baculoviral infection of mammalian cells
• Depositing Lab: Stephen Brohawn
• Publication: Kern et al bioRxiv [Preprint]. 2021 Jan 26:2020.06.17.156554. doi: 10.1101/2020.06.17.156554.

Backbone

• Vector backbone: Derived from pEG BacMam
• Backbone manufacturer: Eric Gouaux Lab
• Vector type: Mammalian Expression
• Selectable markers: Gentamicin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: SARS-CoV-2 3a protein (aa 42-275)
• Species: SARS-CoV-2
• Mutation: aa 42-275
• Promoter: CMV
• Tag / Fusion Protein:
  • EGFP-His (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: CMV Forward
• 3′ sequencing primer: EGFP-N

Resource Information

• A portion of this plasmid was derived from a plasmid made by: IDT Gene Block

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://www.biorxiv.org/lookup/doi/10.1101/2020.06.17.156554 for bioRxiv preprint.