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Addgene

pSBL_DK187
(Plasmid #156423)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 156423 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    Derived from pACEBAC1
  • Backbone manufacturer
    Geneva Biotech
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Gentamicin, 10 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SARS-CoV-2 3a protein
  • Species
    SARS-CoV-2
  • Promoter Polyhedrin
  • Tag / Fusion Protein
    • sfGFP-His (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer pFASTBAC Forward
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    IDT Gene Block

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSBL_DK187 was a gift from Stephen Brohawn (Addgene plasmid # 156423 ; http://n2t.net/addgene:156423 ; RRID:Addgene_156423)

  • For your References section:

    Cryo-EM structure of the SARS-CoV-2 3a ion channel in lipid nanodiscs. Kern DM, Sorum B, Mali SS, Hoel CM, Sridharan S, Remis JP, Toso DB, Kotecha A, Bautista DM, Brohawn SG. bioRxiv [Preprint]. 2021 Jan 26:2020.06.17.156554. doi: 10.1101/2020.06.17.156554. 10.1101/2020.06.17.156554 PubMed 32587976
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