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Addgene

pCAGGS-SARS2-S-D614 (NC-flag)
(Plasmid #156418)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 156418 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCAGGS
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SARS-CoV-2 S protein
  • Species
    SARS CoV2
  • Insert Size (bp)
    3900
  • Entrez Gene
    S (a.k.a. GU280_gp02, spike glycoprotein)
  • Promoter CMV
  • Tags / Fusion Proteins
    • Flag (N terminal on insert)
    • Flag (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site SbfI (not destroyed)
  • 5′ sequencing primer CMV-F
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Pseudotypes onto the MLV vector but not efficiently to VSV or lentiviral vectors

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCAGGS-SARS2-S-D614 (NC-flag) was a gift from Hyeryun Choe & Michael Farzan (Addgene plasmid # 156418 ; http://n2t.net/addgene:156418 ; RRID:Addgene_156418)

  • For your References section:

    SARS-CoV-2 spike-protein D614G mutation increases virion spike density and infectivity. Zhang L, Jackson CB, Mou H, Ojha A, Peng H, Quinlan BD, Rangarajan ES, Pan A, Vanderheiden A, Suthar MS, Li W, Izard T, Rader C, Farzan M, Choe H. Nat Commun. 2020 Nov 26;11(1):6013. doi: 10.1038/s41467-020-19808-4. 10.1038/s41467-020-19808-4 PubMed 33243994
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