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JDW 829 (pTol2-kdrl:V5-mTagBFP2-hsKRAS4A-G12D-ac/Y)
(Plasmid #156415)

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Item Catalog # Description Quantity Price (USD)
Plasmid 156415 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDEST_Tol2
  • Backbone size w/o insert (bp) 12248
  • Vector type
    Zebrafish Transgenesis

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    human mutant KRAS4A-G12D
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1417
  • Mutation
    mutant KRAS4A-G12D
  • GenBank ID
    NM_033360.4
  • Entrez Gene
    KRAS (a.k.a. 'C-K-RAS, C-K-RAS, CFC2, K-RAS2A, K-RAS2B, K-RAS4A, K-RAS4B, K-Ras, K-Ras 2, KI-RAS, KRAS1, KRAS2, NS, NS3, OES, RALD, RASK2, c-Ki-ras, c-Ki-ras2)
  • Promoter kdrl
  • Tag / Fusion Protein
    • mTagBFP2 (N terminal on insert)

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ sequencing primer V5 REV: 5'-CGTAGAATCGAGACCGAGG; V5 FWD: 5'-GGTAAGCCTATCCCTAACCC
  • 3′ sequencing primer KRAS REV2: ctgtattgtcggatctccctc; KRAS FWD2: 5'-gagggagatccgacaatacag;
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    JDW 829 (pTol2-kdrl:V5-mTagBFP2-hsKRAS4A-G12D-ac/Y) was a gift from Joshua Wythe (Addgene plasmid # 156415 ; http://n2t.net/addgene:156415 ; RRID:Addgene_156415)

  • For your References section:

    Somatic Gain of KRAS Function in the Endothelium is Sufficient to Cause Vascular Malformations that Require MEK but not PI3K Signaling. Fish JE, Flores-Suarez CP, Boudreau E, Herman AM, Gutierrez MC, Gustafson D, DiStefano PV, Cui M, Chen Z, Berman De Ruiz K, Schexnayder TS, Ward CS, Radovanovic I, Wythe JD. Circ Res. 2020 Jun 17. doi: 10.1161/CIRCRESAHA.119.316500. 10.1161/CIRCRESAHA.119.316500 PubMed 32552404
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