JDW 295 (pDONR-Dll4-F2-ETS#2-WT-8X)
(Plasmid
#156412)
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PurposeGateway middle entry clone containing an 8X multimerized ETS#2 DNA binding site from the murine Dll4 intronic enhancer
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 156412 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepDONR221
- Backbone size w/o insert (bp) 2545
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Vector typeGateway Cloning
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Stbl3
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemurine Dll4 F2- ETS site B/ site #2, 8X
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SpeciesM. musculus (mouse)
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Insert Size (bp)123
Cloning Information
- Cloning method Gateway Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
JDW 295 (pDONR-Dll4-F2-ETS#2-WT-8X) was a gift from Joshua Wythe (Addgene plasmid # 156412 ; http://n2t.net/addgene:156412 ; RRID:Addgene_156412) -
For your References section:
Somatic Gain of KRAS Function in the Endothelium is Sufficient to Cause Vascular Malformations that Require MEK but not PI3K Signaling. Fish JE, Flores-Suarez CP, Boudreau E, Herman AM, Gutierrez MC, Gustafson D, DiStefano PV, Cui M, Chen Z, Berman De Ruiz K, Schexnayder TS, Ward CS, Radovanovic I, Wythe JD. Circ Res. 2020 Jun 17. doi: 10.1161/CIRCRESAHA.119.316500. 10.1161/CIRCRESAHA.119.316500 PubMed 32552404