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JDW 837 (pCS2-V5-mScarlet-I-hsKRAS4A-G12V)
(Plasmid #156410)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 156410 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCS2-DEST
  • Backbone size w/o insert (bp) 5600
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    human mutant KRAS4A-G12V
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1399
  • Mutation
    mutant KRAS4A-G12V
  • GenBank ID
    NM_033360.4
  • Entrez Gene
    KRAS (a.k.a. 'C-K-RAS, C-K-RAS, CFC2, K-RAS2A, K-RAS2B, K-RAS4A, K-RAS4B, K-Ras, K-Ras 2, KI-RAS, KRAS1, KRAS2, NS, NS3, OES, RALD, RASK2, c-Ki-ras, c-Ki-ras2)
  • Promoter CMV
  • Tag / Fusion Protein
    • mScarlet-I (N terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    JDW 837 (pCS2-V5-mScarlet-I-hsKRAS4A-G12V) was a gift from Joshua Wythe (Addgene plasmid # 156410 ; http://n2t.net/addgene:156410 ; RRID:Addgene_156410)

  • For your References section:

    Somatic Gain of KRAS Function in the Endothelium is Sufficient to Cause Vascular Malformations that Require MEK but not PI3K Signaling. Fish JE, Flores-Suarez CP, Boudreau E, Herman AM, Gutierrez MC, Gustafson D, DiStefano PV, Cui M, Chen Z, Berman De Ruiz K, Schexnayder TS, Ward CS, Radovanovic I, Wythe JD. Circ Res. 2020 Jun 17. doi: 10.1161/CIRCRESAHA.119.316500. 10.1161/CIRCRESAHA.119.316500 PubMed 32552404
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