JDW 827 (pCS2-V5-mTagBFP2-hsKRAS4A-G12D)
(Plasmid #156409)

• Purpose: pCS2 CMV expression vector driving V5-tagged mTagBFP2 fused human KRAS4A-G12D
• Depositing Lab: Joshua Wythe
• Publication: Fish et al Circ Res. 2020 Jun 17. doi: 10.1161/CIRCRESAHA.119.316500.

Backbone

• Vector backbone: pCS2-DEST
• Backbone size w/o insert (bp): 5600
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: human mutant KRAS4A-G12D
• Species: H. sapiens (human)
• Insert Size (bp): 1420
• Mutation: mutant KRAS4A-G12D
• GenBank ID: NM_033360.4
• Entrez Gene: KRAS (a.k.a. 'C-K-RAS, C-K-RAS, CFC2, K-RAS2A, K-RAS2B, K-RAS4A, K-RAS4B, K-Ras, K-Ras 2, KI-RAS, KRAS1, KRAS2, NS, NS3, OES, RALD, RASK2, c-Ki-ras, c-Ki-ras2)
• Promoter: CMV
• Tag / Fusion Protein:
  • mTagBFP2 (N terminal on insert)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: SP6
• 3′ sequencing primer: T3

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Evrogen Limited Use Label License for FPs
• Industry Terms:
  • Not Available to Industry