pNK141g2
(Plasmid
#156380)
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PurposeExpresses orthogonal RBS mPapaya reporter
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 156380 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepACYC184
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Vector typeSynthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Turbos
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemPapaya
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SpeciesSynthetic
Cloning Information
- Cloning method Ligation Independent Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pNK141g2 was a gift from Ahmed Badran (Addgene plasmid # 156380 ; http://n2t.net/addgene:156380 ; RRID:Addgene_156380) -
For your References section:
Orthogonal translation enables heterologous ribosome engineering in E. coli. Kolber NS, Fattal R, Bratulic S, Carver GD, Badran AH. Nat Commun. 2021 Jan 26;12(1):599. doi: 10.1038/s41467-020-20759-z. 10.1038/s41467-020-20759-z PubMed 33500394