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Addgene

pNK083d
(Plasmid #156367)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 156367 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSC101
  • Vector type
    Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Turbos
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    S20, S16 ribosomal genes
  • Species
    P. aeruginosa

Cloning Information

  • Cloning method Ligation Independent Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pNK083d was a gift from Ahmed Badran (Addgene plasmid # 156367 ; http://n2t.net/addgene:156367 ; RRID:Addgene_156367)

  • For your References section:

    Orthogonal translation enables heterologous ribosome engineering in E. coli. Kolber NS, Fattal R, Bratulic S, Carver GD, Badran AH. Nat Commun. 2021 Jan 26;12(1):599. doi: 10.1038/s41467-020-20759-z. 10.1038/s41467-020-20759-z PubMed 33500394
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