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pInducer20_SLC38A2_N82A
(Plasmid #156183)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 156183 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pInducer20
  • Backbone size w/o insert (bp) 11000
  • Total vector size (bp) 11669
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SLC38A2
  • Alt name
    SNAT2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1521
  • Mutation
    Silent mutations to prevent targeting by sgRNA: TAATCTGAGCAATGCGATTG. N82A (AAT --> GCT) "transport defective" mutation.
  • Entrez Gene
    SLC38A2 (a.k.a. ATA2, PRO1068, SAT2, SNAT2)
  • Promoter TRE

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pInducer20_SLC38A2_N82A was a gift from Alec Kimmelman (Addgene plasmid # 156183 ; http://n2t.net/addgene:156183 ; RRID:Addgene_156183)

  • For your References section:

    Selective alanine transporter utilization creates a targetable metabolic niche in pancreatic cancer. Parker SJ, Amendola CR, Hollinshead KER, Yu Q, Yamamoto K, Encarnacion-Rosado J, Rose RE, LaRue MM, Sohn ASW, Biancur DE, Paulo JA, Gygi SP, Jones DR, Wang H, Philips MR, Bar-Sagi D, Mancias JD, Kimmelman AC. Cancer Discov. 2020 Apr 27. pii: 2159-8290.CD-19-0959. doi: 10.1158/2159-8290.CD-19-0959. 10.1158/2159-8290.CD-19-0959 PubMed 32341021
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